Novel methylation specific real-time PCR test for the diagnosis of Klinefelter syndrome
نویسندگان
چکیده
منابع مشابه
Novel methylation specific real-time PCR test for the diagnosis of Klinefelter syndrome
The aim of this study was to design a molecular assay for the diagnosis of Klinefelter syndrome (KS), based on the detection of supernumerary X-chromosomes (X-chs). DNA was extracted from peripheral blood samples of twenty-six 47,XXY males; two 46,XY/47,XXY males; twenty-two 46,XY males; and 15 females; and deaminated. Methylation-specific quantitative polymerase chain reaction (MS-qPCR) was pe...
متن کاملMethylation-Specific Fluorescent Amplicon Generation, a Novel Real-Time Signal Generation Method for Methylation-Specific PCR
Background: Aberrant promoter methylation is a major mechanism for silencing tumor suppressor genes in cancer. Detection of hypermethylation is used as a molecular marker for early cancer diagnosis, as a prognostic index, or to define therapeutic targets for reversion of aberrant methylation. We report on a novel signal generation technology for real-time PCR to detect gene promoter methylation...
متن کاملuse of real-time pcr method on the amniocyte samples for prenatal diagnosis of down syndrome
objective: in this study, the possibility of prenatal diagnosis of down syndrome with real-time pcr method was evaluated. in this context, optimization of a suitable method for purification of high quality dna from amniotic fluid samples was also considered. materials and methods: pregnant women who had the high risk of having babies with down syndrome were selected according to the biochemica...
متن کاملMS-FLAG, a novel real-time signal generation method for methylation-specific PCR.
BACKGROUND Aberrant promoter methylation is a major mechanism for silencing tumor suppressor genes in cancer. Detection of hypermethylation is used as a molecular marker for early cancer diagnosis, as a prognostic index, or to define therapeutic targets for reversion of aberrant methylation. We report on a novel signal generation technology for real-time PCR to detect gene promoter methylation....
متن کاملNovel real-time quantitative PCR test for trisomy 21.
method correlated well with the other two compared hs-CRP methods in the range of 0.2–100 mg/L. At concentrations 100 mg/L, however, poor correlations were found between all compared methods. Because we are convinced that for the clinical laboratory it is most practical to use one CRP method for the complete measuring range, further investigation to improve agreement among the different methods...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Asian Journal of Andrology
سال: 2014
ISSN: 1008-682X
DOI: 10.4103/1008-682x.125914